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KMID : 0379120130410010009
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Korean Journal of Mycology
2013 Volume.41 No. 1 p.9 ~ p.13
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Identification of Orchid Mycorrhizal Fungi Isolated from Epipactis thunbergii in Korea
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Han Han-Kyeol
Chung Jae-Min
Cho Yong-Chan
Kim Dae-Shin
Eom Ahn-Heum
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Abstract
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In this study, roots of Epipactis thunbergii were collected from Chujado on the north of Jeju-do. Six fungal isolates were isolated from surface-sterilized roots of the orchid and classified with groups based on morphological characteristics. Fungal DNA was extracted from each isolate and amplified ITS region using ITS1-OF/ITS4-OF primer pair. Three species of orchid mycorrhizal fungi were identified as Tulasnella calospora, Tulasnella sp. and Sebacina sp. based on molecular and morphological characteristics.
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KEYWORD
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Epipactis thunbergii, Orchid mycorrhizal fungi, Sebacina, Tulasnella calospora
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